|
Sign In to gain access to subscriptions and/or personal tools.
|
Total Parenteral Nutrition in the Mouse: Development of a Technique
Harry S. Sitren, PH.D.
Food Science and Human Nutrition Department and Department of Medicine, University of Florida, Gainesville, Florida
Patricia A. Heller, B.S.
Food Science and Human Nutrition Department and Department of Medicine, University of Florida, Gainesville, Florida
Lynn B. Bailey, PH.D.
Food Science and Human Nutrition Department and Department of Medicine, University of Florida, Gainesville, Florida
James J. Cerda, M.D.
Food Science and Human Nutrition Department and Department of Medicine, University of Florida, Gainesville, Florida
A method for total parenteral nutrition in the mouse was developed using commercially available supplies and equipment. The mouse's inferior vena cava was catheterized and the catheter was exteriorized from the tail. Mice (average body weight 22.5 g) were not tethered but instead were partially restrained by immobilizing the tail to protect the infusion tubing. A solution was formulated to contain 40% dextrose and 4.3% amino acids plus vitamins, electrolytes, and trace elements. It was administered via pump in graded fashion for the first 3 days to allow the mice to adapt, and then at a rate of 8 ml/day thereafter. This volume provided approximately 12 kcal and 54 mg nitrogen per day and was calculated to meet the needs of the mouse fed per os according to guidelines of the National Research Council. During the adaptation period mice lost weight but they were in positive N balance thereafter. At the end of 12 days body weight was not different than at the start, indicating the adequacy of nutrient input. Further, there were no differences in nitrogen balance or body weight in total parenteral nutrition fed mice in comparison with tail-restrained mice given the same solution per os. All mice appeared to tolerate the partial restraint without incidence and showed no untoward side effects. The rationale and validity of this technique is discussed in detail. (Journal of Parenteral and Enteral Nutrition 7:582-586, 1983)
Journal of Parenteral and Enteral Nutrition, Vol. 7, No. 6,
582-586 (1983)
DOI: 10.1177/0148607183007006582
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Omata, K. Fukatsu, S. Murakoshi, M. Noguchi, H. Miyazaki, T. Moriya, K. Okamoto, S. Fukazawa, T. Akase, D. Saitoh, et al.
Enteral Refeeding Rapidly Restores PN-Induced Reduction of Hepatic Mononuclear Cell Number Through Recovery of Small Intestine and Portal Vein Blood Flows
JPEN J Parenter Enteral Nutr,
November 1, 2009;
33(6):
618 - 626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Hermsen, F. E. Gomez, Y. Maeshima, Y. Sano, W. Kang, and K. A. Kudsk
Decreased Enteral Stimulation Alters Mucosal Immune Chemokines
JPEN J Parenter Enteral Nutr,
January 1, 2008;
32(1):
36 - 44.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Kudsk, F. E. Gomez, W. Kang, and C. Ueno
Enteral Feeding of a Chemically Defined Diet Preserves Pulmonary Immunity but Not Intestinal Immunity: The Role of Lymphotoxin {beta} Receptor
JPEN J Parenter Enteral Nutr,
November 1, 2007;
31(6):
477 - 481.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sano, F. E. Gomez, W. Kang, J. Lan, Y. Maeshima, J. L. Hermsen, C. Ueno, and K. A. Kudsk
Intestinal Polymeric Immunoglobulin Receptor Is Affected by Type and Route of Nutrition
JPEN J Parenter Enteral Nutr,
September 1, 2007;
31(5):
351 - 357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Kang, K. A. Kudsk, Y. Sano, J. Lan, F. Yang-Xin, F. E. Gomez, and Y. Maeshima
Effects of Lymphotoxin {beta} Receptor Blockade on Intestinal Mucosal Immunity
JPEN J Parenter Enteral Nutr,
September 1, 2007;
31(5):
358 - 365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Maeshima, K. Fukatsu, T. Moriya, F. Ikezawa, C. Ueno, D. Saitoh, and H. Mochizuki
Influence of Adding Fish Oil to Parenteral Nutrition on Gut-Associated Lymphoid Tissue
JPEN J Parenter Enteral Nutr,
September 1, 2007;
31(5):
416 - 422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. E. Gomez, J. Lan, W. Kang, C. Ueno, and K. A. Kudsk
Parenteral Nutrition and Fasting Reduces Mucosal Addressin Cellular Adhesion Molecule-1 (MAdCAM-1) mRNA in Peyer's Patches of Mice
JPEN J Parenter Enteral Nutr,
January 1, 2007;
31(1):
47 - 52.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fukatsu, T. Moriya, F. Ikezawa, Y. Maeshima, J. Omata, Y. Yaguchi, K. Okamoto, H. Mochizuki, H. Hiraide, and G. Hardy
Interleukin-7 Dose-Dependently Restores Parenteral Nutrition-Induced Gut-Associated Lymphoid Tissue Cell Loss but Does Not Improve Intestinal Immunoglobulin A Levels
JPEN J Parenter Enteral Nutr,
September 1, 2006;
30(5):
388 - 394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Marini, B. Lee, and P. J. Garlick
Reduced Ornithine Transcarbamylase Activity Does Not Impair Ureagenesis in Otcspf-ash Mice
J. Nutr.,
April 1, 2006;
136(4):
1017 - 1020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. G. Murali, D. W. Nelson, A. K. Draxler, X. Liu, and D. M. Ney
Insulin-Like Growth Factor-I (IGF-I) Attenuates Jejunal Atrophy in Association with Increased Expression of IGF-I Binding Protein-5 in Parenterally Fed Mice
J. Nutr.,
November 1, 2005;
135(11):
2553 - 2559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Genton, K. A. Kudsk, S. R. Reese, and S. Ikeda
Enteral Feeding Preserves Gut Th-2 Cytokines Despite Mucosal Cellular Adhesion Molecule-1 Blockade
JPEN J Parenter Enteral Nutr,
January 1, 2005;
29(1):
44 - 47.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fukatsu, A. H. Lundberg, M. K. Hanna, Y. Wu, H. G. Wilcox, D. N. Granger, A. O. Gaber, and K. A. Kudsk
Increased Expression of Intestinal P-Selectin and Pulmonary E-Selectin During Intravenous Total Parenteral Nutrition
Arch Surg,
October 1, 2000;
135(10):
1177 - 1182.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Hanna, B. L. Zarzaur Jr, K. Fukatsu, R. Chance DeWitt, K. B. Renegar, C. Sherrell, Y. Wu, and K. A. Kudsk
Individual Neuropeptides Regulate Gut-Associated Lymphoid Tissue Integrity, Intestinal Immunoglobulin A Levels, and Respiratory Antibacterial Immunity
JPEN J Parenter Enteral Nutr,
September 1, 2000;
24(5):
261 - 269.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. A. Kudsk, Y. Wu, K. Fukatsu, B. L. Zarzaur, C. D. Johnson, R. Wang, and M. K. Hanna
Glutamine-Enriched Total Parenteral Nutrition Maintains Intestinal Interleukin-4 and Mucosal Immunoglobulin A Levels
JPEN J Parenter Enteral Nutr,
September 1, 2000;
24(5):
270 - 275.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. Fukatsu, A. H. Lundberg, M. K. Hanna, Y. Wu, H. G. Wilcox, D. N. Granger, A. O. Gaber, and K. A. Kudsk
Route of Nutrition Influences Intercellular Adhesion Molecule-1 Expression and Neutrophil Accumulation in Intestine
Arch Surg,
October 1, 1999;
134(10):
1055 - 1060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Li, B. K. King, P. G. Janu, K. B. Renegar, and K. A. Kudsk
Glycyl-L-Glutamine-Enriched Total Parenteral Nutrition Maintains Small Intestine Gut-Associated Lymphoid Tissue and Upper Respiratory Tract Immunity
JPEN J Parenter Enteral Nutr,
January 1, 1998;
22(1):
31 - 36.
[Abstract]
[PDF]
|
|
|
|
|
|
|
B. K. King, J. Li, and K. A. Kudsk
A Temporal Study of TPN-Induced Changes in Gut-Associated Lymphoid Tissue and Mucosal Immunity
Arch Surg,
December 1, 1997;
132(12):
1303 - 1309.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. O. Castillo, A. Pittler, and F. Costa
Intestinal Maturation in the Rat: The Role of Enteral Nutrients
JPEN J Parenter Enteral Nutr,
September 1, 1988;
12(5):
490 - 495.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E. M. Copeland III
Jonathan E. Rhoads lecture. Intravenous Hyperalimentation and Cancer. A Historical Perspective
JPEN J Parenter Enteral Nutr,
July 1, 1986;
10(4):
337 - 342.
[PDF]
|
|
|
|
|
|
|
H. S. Sitren, P. A. Heller, L. B. Bailey, T. G. Baumgartner, and J. J. Cerda
Total Parenteral Nutrition in the Mouse: Body Composition and Plasma Chemistries
JPEN J Parenter Enteral Nutr,
September 1, 1985;
9(5):
600 - 604.
[Abstract]
[PDF]
|
|
|
|
|
