This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Schiffrin, E. J. Articles by Breuillé, D. Search for Related Content PubMed PubMed Citation Articles by Schiffrin, E. J. Articles by Breuillé, D. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Crohn's Disease Social Bookmarking                   What's this?

Milk Casein-Based Diet Containing TGF-β Controls the Inflammatory Reaction in the HLA-B27 Transgenic Rat Model

Eduardo J. Schiffrin, MD^*, Mimoun El Yousfi, PhD, Magali Faure, PhD^*, Lydia Combaret, PhD, Anne Donnet, PhD^*, Stephanie Blum, PhD^*, Christiane Obled, PhD and Denis Breuillé, PhD^*

From the ^* Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland; and the Unité de Nutrition et Métabolisme Protéique, INRA, Ceyrat, France

Correspondence: Eduardo J Schiffrin, Nestlé Nutrition, Ave Nestlé 15, 1800 Vevey, Switzerland. Electronic mail may be sent to eduardo.schiffrin{at}rdls.nestle.com.

Background: A casein-based formula containing TGF-β has been successfully used in adolescents during acute episodes of Crohn's disease. The role played by this molecule requires confirmation. We have examined the capacity of a TGF-β containing diet to control the intestinal inflammation in HLA-B27 transgenic rats, and compared its effects with a similar diet devoid of TGF-β. Methods: Three groups of rats were studied. HLA-B27/hβ2M transgenic rats were fed with a casein-based rat-adapted diet containing TGF-β or a control casein-based diet without TGF-β. Fischer control animals were fed the latter. Body weight, dietary intake, tissue weights, fecal samples, leukocyte counts, and acute phase response were analyzed. Intestinal inflammation was assessed by histology, myeloperoxidase, and mRNA expression of cytokines. MUC2 protein expression was assessed by immunohistochemistry. Breakdown of muscle protein was examined. Results: The test diet improved diarrhea increasing the fecal dry matter and the colonic inflammation as shown by a lower inflammatory score (2.43 ± 1.13 vs 4.42 ± 0.53, p < .05), lower mucosal thickness (431.25 ± 72.29 vs 508.57 ± 81.32 µm, p = .08) and decreased IFN mRNA expression. MUC2 protein expression was increased in HLA rats fed the TGF-β diet compared with HLA rats fed the control diet, but restitution to normal pattern was not observed. The test diet also decreased leukocytosis and the acute phase response and improved the muscle catabolic response. Conclusion: The TGF-β containing diet has a beneficial effect in an animal model of intestinal inflammation. Our observations support a potential role for dietary TGF-β in the restoration of immune homeostasis.

Discussion

Journal of Parenteral and Enteral Nutrition, Vol. 29, No. 4 suppl, S141-S150 (2005)
DOI: 10.1177/01486071050290S4S141


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Faure, C. Mettraux, D. Moennoz, J.-P. Godin, J. Vuichoud, F. Rochat, D. Breuille, C. Obled, and I. Corthesy-Theulaz Specific Amino Acids Increase Mucin Synthesis and Microbiota in Dextran Sulfate Sodium-Treated Rats J. Nutr., June 1, 2006; 136(6): 1558 - 1564. [Abstract] [Full Text] [PDF]