| Sign In to gain access to subscriptions and/or personal tools. |
Enteral Feeding Preserves Gut Th-2 Cytokines Despite Mucosal Cellular Adhesion Molecule-1 Blockade
From the * Veterans Administration Surgical
Services, William S. Middleton Memorial Veterans Hospital Madison, Wisconsin;
and the Correspondence: Kenneth A. Kudsk, MD, University of Wisconsin Medical School, Department of Surgery, H4/730 Clinical Science Center, 600 Highland Avenue, Madison, WI 53792-7375. Electronic mail may be sent to kudsk{at}surgery.wisc.edu.
Background: Parenteral nutrition (PN) decreases gut-associated lymphoid tissue (GALT), the intestinal IgA stimulating cytokines IL-4 and IL-10 in gut homogenates, intestinal IgA levels and the expression of Peyer patch (PP) mucosal cellular adhesion molecule-1 (MAdCAM-1), an adhesion molecule found on the high endothelial venules of PP and other tissues. IL-4 in PP stimulates MAdCAM expression in vitro. MAdCAM-1 blockade with MECA-367 reduces GALT cell populations to PN levels but maintains intestinal IgA levels if the animals are chow fed. This study compares IL-4 levels in PP of chow and PN fed mice and measures the effects of MAdCAM blockade on IL-4 and IL-10 levels in gut homogenates of chow fed mice. We hypothesized that in vivo IL-4 levels drop in PP of PN fed mice and IL-4 and IL-10 levels are maintained after MAdCAM-1 blockade in chow fed mice. Methods: Exp 1: 18 mice received chow or PN for 5 days to determine PP IL-4 levels. Exp 2: 44 mice were randomized to chow + control monoclonal antibody (mAb), chow + MECA-367 (anti-MAdCAM-1 mAb) or PN for 4 days before measurement of IL-4 and IL-10 levels in gut homogenates. Results: Exp 1: IL-4 levels in vivo were lower in PP of PN-fed mice than chow fed mice (92.0 ± 15.1 pg/mL vs 251.1 ± 14.8, p = .0003). Exp 2: IL-4 levels were significantly higher in chow + control mAb (187.1 ± 44.1 pg/mL) and chow + MECA-367 (110.9 ± 19.1 pg/mL) groups than PN mice (21.8 ± 30.6 pg/mL, p < .02 vs chow + control or chow + MECA-367). IL-10 levels were significantly lower with PN (23.1 ± 40.9 pg/mL) with chow+control (174.0 ± 22.2 pg/mL p < .01), or chow + MECA-367 (181.7 ± 23.1 pg/mL, p < .02 vs PN). Conclusions: PN-feeding reduces in vivo IL-4 levels in PP (consistent with lowered MAdCAM-1 expression) and IL-4 and IL-10 levels in gut homogenates compared with chow. Despite MAdCAM-1 blockade, enteral feeding preserved gut IL-4 levels and increased IL-10 levels consistent with preserved IgA levels.
Journal of Parenteral and Enteral Nutrition, Vol. 29, No. 1,
44-47 (2005) This article has been cited by other articles:
|
|
|||||||||||||||||||||||||||
 |
|
|
 |
|
Sign In to gain access to subscriptions and/or personal tools.
