This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Lin, M.-T. Articles by Chen, W.-J. Search for Related Content PubMed PubMed Citation Articles by Lin, M.-T. Articles by Chen, W.-J. Social Bookmarking                         What's this?

Alanyl-Glutamine Enriched Total Parenteral Nutrition Improves Local, Systemic, and Remote Organ Responses to Intraperitoneal Bacterial Challenge

Department of Surgery, National Taiwan University, Taipei, Taiwan, Department of Surgery, University of Tokyo, Japan, linmt{at}ha.mc.ntu.edu.tw

Hideaki Saito, MD, PhD

Department of Surgery, University of Tokyo, Japan

Satoshi Furukawa, MD

Department of Surgery, University of Tokyo, Japan

Ryoji Fukushima, MD, PhD

Department of Surgery, University of Tokyo, Japan

Fukatsu Kazuhiko, MD, PhD

Department of Surgery, University of Tokyo, Japan

Po-Huang Lee, MD, PhD

Department of Surgery, National Taiwan University, Taipei, Taiwan

King-Jen Chang, MD, PhD

Department of Surgery, National Taiwan University, Taipei, Taiwan

Wei-Jao Chen, MD, PhD

Department of Surgery, National Taiwan University, Taipei, Taiwan

Background: Standard total parenteral nutrition (STD-TPN) may diminish host defense against infection. Glutamine (Gln) is suggested to enhance host immunity. This study investigated the effects of antecedent alanyl-glutamine enriched TPN (Ala-Gln-TPN) on host responses to intraperitoneal bacterial challenge compared with STD-TPN. Methods: Rats were divided into STD-TPN and Ala-Gln-TPN groups. They received isocaloric and isonitrogenous nutrition for 7 days and were challenged intraperitoneally with E. coli. Rats were killed before (0 hour) challenge and at 2 and 6 hours after challenge. Bacterial numbers in peritoneal lavage fluid (PLF), liver, spleen, and blood were determined. Tumor necrosis factor- (TNF), interleukin (IL)-8, and interferon- (IFN) in plasma and PLF were measured. Hepatic TNF, splenic TNF, and splenic IFN levels were determined. Results: The numbers of E. coli in systemic blood at 2 hours after intraperitoneal bacterial challenge were significantly lower in the Ala-Gln-TPN than in STD-TPN group. E. coli numbers in blood significantly correlated with those in the liver. The Ala-Gln-TPN also resulted in significantly higher PLF and hepatic TNF levels, higher splenic IFN levels, and lower plasma IL-8 levels at 6 hours after challenge compared with the STD-TPN. Conclusions: Antecedent Ala-Gln enriched TPN enhance local, systemic, and remote organ immune responses to intraperitoneal bacterial challenge. Ala-Gln-TPN may enhance host defense and be more beneficial than standard TPN in sepsis. (journal of Parenteral and Enteral Nutrition 25:346-351, 2001)

Journal of Parenteral and Enteral Nutrition, Vol. 25, No. 6, 346-351 (2001)
DOI: 10.1177/0148607101025006346


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M.-Y. Wang, M.-H. Wu, D.-Y. Hsieh, L.-J. Lin, P.-H. Lee, W.-J. Chen, and M.-T. Lin Home Parenteral Nutrition Support in Adults: Experience of a Medical Center in Asia JPEN J Parenter Enteral Nutr, July 1, 2007; 31(4): 306 - 310. [Abstract] [Full Text] [PDF]

				R. Yang, X. Tan, A. M. Thomas, R. Steppacher, N. Qureshi, D. C. Morrison, and C. W. Van Way III Alanine-Glutamine Dipeptide (AGD) Inhibits Expression of Inflammation-Related Genes in Hemorrhagic Shock JPEN J Parenter Enteral Nutr, January 1, 2007; 31(1): 32 - 36. [Abstract] [Full Text] [PDF]

				A. Hubert-Buron, J. Leblond, A. Jacquot, P. Ducrotte, P. Dechelotte, and M. Coeffier Glutamine Pretreatment Reduces IL-8 Production in Human Intestinal Epithelial Cells by Limiting I{kappa}B{alpha} Ubiquitination J. Nutr., June 1, 2006; 136(6): 1461 - 1465. [Abstract] [Full Text] [PDF]

				E. V. Bobrovnikova-Marjon, P. L. Marjon, O. Barbash, D. L. Vander Jagt, and S. F. Abcouwer Expression of Angiogenic Factors Vascular Endothelial Growth Factor and Interleukin-8/CXCL8 Is Highly Responsive to Ambient Glutamine Availability: Role of Nuclear Factor-{kappa}B and Activating Protein-1 Cancer Res., July 15, 2004; 64(14): 4858 - 4869. [Abstract] [Full Text] [PDF]

				L. Castell, C. Vance, R. Abbott, J. Marquez, and P. Eggleton Granule Localization of Glutaminase in Human Neutrophils and the Consequence of Glutamine Utilization for Neutrophil Activity J. Biol. Chem., April 2, 2004; 279(14): 13305 - 13310. [Abstract] [Full Text] [PDF]