This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Geller, D. A. Articles by Murase, N. Search for Related Content PubMed PubMed Citation Articles by Geller, D. A. Articles by Murase, N. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB (L)-ARGININE L-LYSINE NITRIC OXIDE Medline Plus Health Information Liver Transplantation Social Bookmarking                         What's this?

Protective Role of the L-Arginine-Nitric Oxide Synthase Pathway on Preservation Injury after Rat Liver Transplantation

Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, gellerda{at}msx.upmc.edu.edu

Stanley H. Chia, MD

Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania

Yoshihito Takahashi, MD

Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania

Gautam P. Yagnik, BS

Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania

George Tsoulfas, MD

Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania

Noriko Murase, MD

Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania

Background : A major problem complicating liver transplantation is the preservation injury that results from cold storage and subsequent ischemia/reperfusion injury after organ revascularization. The L—arginine—nitric oxide (NO) pathway has been recognized to play critical roles during infection, inflammation, organ injury, and transplant rejection. Recent data indicates that NO synthesis has beneficial effects in several models of liver injury. The purpose of this study is to examine the role of the L—arginine—NO pathway on preservation injury in an experimental model of rat liver transplantation. Methods: Orthotopic liver transplantation was performed in syngeneic (LEW to LEW) rats. Liver preservation injury was determined by measuring serum liver function tests 6 to 48 hours after transplantation. In some experiments, rats received L-arginine supplementation 0 to 24 hours after transplantation. In other experiments, NO synthase inhibitors (L-NAME or L-NIL) were injected at the time of isograft revascularization. Results: L-Arginine supplementation decreased hepatic transaminase levels at all time points examined (6-48 hours). L-Arginine produced a significant improvement in liver preservation injury by 12 hours after reperfusion. The NO synthase inhibitor L-NAME caused a significant increase in liver injury 24 hours after injection. The inducible NO synthase (iNOS)-specific inhibitor L-NIL had no significant effect on liver injury. Conclusions: The results show that L-arginine supplementation and NO synthesis improve hepatic injury and have a protective role in the transplanted liver graft. The protective effect may be mediated by low-level cNOS-derived NO. (Journal of Parenteral and Enteral Nutrition 25:142-147, 2001)

Journal of Parenteral and Enteral Nutrition, Vol. 25, No. 3, 142-147 (2001)
DOI: 10.1177/0148607101025003142


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K. M. Reid, A. Tsung, T. Kaizu, G. Jeyabalan, A. Ikeda, L. Shao, G. Wu, N. Murase, and D. A. Geller Liver I/R injury is improved by the arginase inhibitor, N{omega}-hydroxy-nor-L-arginine (nor-NOHA) Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G512 - G517. [Abstract] [Full Text] [PDF]