This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Cui, X.-L. Articles by Ogoshi, S. Search for Related Content PubMed PubMed Citation Articles by Cui, X.-L. Articles by Ogoshi, S. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB (L)-ARGININE NITRIC OXIDE Medline Plus Health Information Burns Social Bookmarking                         What's this?

Arginine-Supplemented Diet Decreases Expression of Inflammatory Cytokines and Improves Survival in Burned Rats

Department of Surgery, Nankoku, Kochi, Japan

Masato Iwasa, MD, PhD

Department of Surgery, Nankoku, Kochi, Japan

Yoshie Iwasa, MD, PhD

Department of Surgery, Nankoku, Kochi, Japan

Shohei Ogoshi, MD, PhD

Kochi Medical School, Nankoku, Kochi, Japan

Background: We examined whether the expression of inflammatory cytokines in organs was influenced by the enteral diet supplemented with arginine in burned rats. Methods: Male Wistar rats weighing about 200 g underwent catheter jejunostomy and received scald burns covering 30% of the whole-body surface area. Animals were divided into two groups: a control group (no supplemental arginine, n = 12) and an arginine group (supplemental arginine: 7.7 g/L, n = 10), which continuously received total enteral nutrition for 7 days (250 kcal/kg/d, 1.72 gN/kg/d). The following were measured after the experiment: (1) messenger RNA (mRNA) expression of tumor necrosis factor-alpha (TNF-), interferon-gamma (IFN-), interleukin-1beta (IL-1β), and IL-6 in the spleen, thymus, lung, and liver by a semiquantitative reverse transcription-polymerase chain reaction method, (2) inflammatory cytokines in the plasma and supernatant of cultured splenic lymphocytes by enzyme-linked immunosorbant assay, (3) nitric oxide (NO) product, NO₂^-/NO₃ ^-, in the plasma and supernatant of cultured splenic lymphocytes by the Griess method, and (4) survival rate by the Kaplan-Meier method. Results: The mRNA expression of TNF- was significantly decreased in the spleen and lung (p < .01, p < .05), IFN- in the lung (p < .05), IL-1β in the spleen (p < .05), and IL-6 in the thymus and liver (p < .05, p < .05) in the arginine group when compared with the control group. The production of TNF- by splenic lymphocytes was suppressed in the arginine group in both concanavalin A (Con A)-treated and -untreated cultures (p < .01, p < .05). The production of IFN- by splenic lymphocytes treated with Con A was suppressed in the arginine group (p < .05). The NO product in the supernatant without Con A was increased in the arginine group (p < .05). The mortality rate of the arginine group (0%) was lower than that in the control group (33.3%) on day 7 after the burn injury (p < .05). Conclusions: The data suggest that dietary arginine supplementation decreases the mRNA expression of inflammatory cytokines in organs and improves the survival rate after thermal injury. (Journal of Parenteral and Enteral Nutrition 24:89-96, 2000)

Journal of Parenteral and Enteral Nutrition, Vol. 24, No. 2, 89-96 (2000)
DOI: 10.1177/014860710002400289


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. G. Jeschke, D. N. Herndon, C. Ebener, R. E. Barrow, and K.-W. Jauch Nutritional Intervention High in Vitamins, Protein, Amino Acids, and {omega}3 Fatty Acids Improves Protein Metabolism During the Hypermetabolic State After Thermal Injury Arch Surg, November 1, 2001; 136(11): 1301 - 1306. [Abstract] [Full Text] [PDF]

				B. Langkamp-Henken, K. A. Herrlinger-Garcia, J. K. Stechmiller, J. A. Nickerson-Troy, B. Lewis, and L. Moffatt Arginine Supplementation Is Well Tolerated but Does Not Enhance Mitogen-Induced Lymphocyte Proliferation in Elderly Nursing Home Residents with Pressure Ulcers JPEN J Parenter Enteral Nutr, September 1, 2000; 24(5): 280 - 287. [Abstract] [PDF]