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Insulin-Like Growth Factor-1 Modulation of Intestinal Epithelial Cell Restitution

Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York

Riichiro Nezu, MD

Department of Surgery, Nissei Hospital, Osaka, Japan

Masafumi Wasa, MD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Kinya Sando, MD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Shinkichi Kamata, MD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Yoji Takagi, MD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Akira Okada, MD

Department of Pediatric Surgery, Osaka University Medical School, Japan

After superficial intestinal injury, the mucosal integrity is reestablished by rapid migration of epithelial cells from the adjacent area in a process called restitution. Our previous study suggested that growth hormone improves intestinal healing in an experimental small bowel ulceration, mediated by insulin-like growth factor-1 (IGF-1). The aim of the present study was to assess the role of IGF-1 in mucosal epithelial restitution using an in vitro epithelial wound model. Wounds were established in confluent monolayers of the intestinal cell line, IEC-6. Migration was quantitated in the presence or absence of IGF-1 as the number of cells migrating across the wound edge. Proliferation was assessed by thymidine incorporation. IGF-1-enhanced epithelial cell migration by 2- to 2.5-fold after 12- and 24-hour treatment, respectively, the first step involved in gastrointestinal wound healing. Cell proliferation was significantly stimulated by IGF-1 as well. In addition, expression of transforming growth factor-β (TGF-β) mRNA was significantly enhanced in the wounded monolayers treated with IGF-1. IGF-1 receptor mRNA was found to be detectable throughtout the gastrointestinal mucosa and in the intestinal epithelial cells. In conclusion, these findings suggest that IGF-1 plays an important role in reconstitution of intestinal epithelial integrity after mucosal injury. (Journal of Parenteral and Enteral Nutrition 23: S89-S92, 1999)

Journal of Parenteral and Enteral Nutrition, Vol. 23, No. 5 Suppl, S89-S92 (1999)
DOI: 10.1177/014860719902300522


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