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Wheat Bran Decreases Aberrant Crypt Foci, Preserves Normal Proliferation, and Increases Intraluminal Butyrate Levels in Experimental Colon Cancer

Harrison Department of Surgical Research, University of Pennsylvania Medical Center, Philadelphia, Department of Bioscience and Biotechnology, Drexel University, Philadelphia. Pennsylvania

Wendy L. Frankel, MD

Department of Surgical Pathology, Ohio State University, Columbus

John Tazelaar, MD

Institute for Human Gene Therapy, University of Pennsylvania, Philadelphia

John A. Lawson

Center for Experimental Therapeutics, University of Pennsylvania, Philadelphia

Shortie McKinney, PhD

Department of Bioscience and Biotechnology, Drexel University, Philadelphia. Pennsylvania

Stanley Segall, PhD

Department of Bioscience and Biotechnology, Drexel University, Philadelphia. Pennsylvania

Bruce P. Kinosian, MD

Department of Surgery, and Statistical Consultant, University of Pennsylvania Medical Center, Philadelphia

Noel N. Williams, MD

Harrison Department of Surgical Research, University of Pennsylvania Medical Center, Philadelphia

John L. Rombeau, MD

Harrison Department of Surgical Research, University of Pennsylvania Medical Center, Philadelphia

Background: Dietary wheat bran protects against colon cancer, but the mechanism(s) of this effect is not known. Butyrate, produced by colonic bacterial fermentation of dietary polysaccharides, such as wheat bran, induces apoptosis and decreases proliferation in colon cancer cell lines. Whether similar effects occur in vivo is not well defined. We hypothesized that wheat bran's antineoplastic effects in vivo may be mediated in part by butyrate's modulation of apoptosis and proliferation. Methods: Male F344 rats were fed wheat bran-supplemented or an isocaloric, isonitrogenous fiber-free diet. Rats were treated with one dose of the carcinogen azoxymethane or vehicle with sacrifice after 5 days (tumor initiation); or two doses (days 0 and 7) with sacrifice after 56 days (tumor promotion). Study variables included fecal butyrate levels and the intermediate biomarkers of colon carcinogenesis, aberrant crypt foci (ACF), and changes in crypt cell proliferation and apoptosis. Results: During tumor initiation, wheat bran produced greater apoptosis (p = .01), a trend toward less proliferation, and preserved the normal zone of proliferation (p = .01). At tumor promotion, wheat bran decreased the number of ACF (proximal colon, p = .005; distal colon, p = .047) and maintained the normal proliferative zone. The fiber-free diet shifted the zone of proliferation into the premalignant pattern in both studies. Wheat bran produced significantly higher fecal butyrate (p = .01; .004, .00001) levels than the fiber-free diet throughout the tumor promotion study. Conclusions: Wheat bran increased apoptosis and controlled proliferation during tumor initiation and resulted in decreased ACF. Wheat bran's antineoplastic effects occurred early after carcinogen exposure, and were associated with increased fecal butyrate levels. (Journal of Parenteral and Enteral Nutrition 23:269-278, 1999)

Journal of Parenteral and Enteral Nutrition, Vol. 23, No. 5, 269-278 (1999)
DOI: 10.1177/0148607199023005269


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