This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Laborie, S. Articles by Chessex, P. Search for Related Content PubMed PubMed Citation Articles by Laborie, S. Articles by Chessex, P. Social Bookmarking                         What's this?

Protecting Solutions of Parenteral Nutrition From Peroxidation

Departments of Pediatrics and Pharmacy, and the Research Center of Hospital Ste-Justine, University of Montreal, Quebec, Canada

Jean-Claude Lavoie, PhD

Departments of Pediatrics and Pharmacy, and the Research Center of Hospital Ste-Justine, University of Montreal, Quebec, Canada

Marjolain Pineault, PhD

Departments of Pediatrics and Pharmacy, and the Research Center of Hospital Ste-Justine, University of Montreal, Quebec, Canada

Philippe Chessex, MD

Departments of Pediatrics and Pharmacy, and the Research Center of Hospital Ste-Justine, University of Montreal, Quebec, Canada

Background : Light exposure induces the generation of peroxides in solutions of total parenteral nutrition (TPN). Peroxide toxicity has been documented in cell, in tissue, and in isolated organs. To decrease the infused peroxide load and to protect the quality of the parenteral nutrients, we tested the photoprotective properties of different infusion sets. Methods: Solutions of fat-free TPN and all-in-one total nutrient admixture (TNA) were run through sets of bags (clear and covered) and tubings (clear and colored: black, orange, and yellow) offering different levels of protection against light. Peroxide levels were determined by ferrous oxidation of xylenol orange, thiol functions by the 5,5,dithiobis(2-nitrobenzoic acid) technique, and absorbance of tubings by spectroscopy. Results: Protection of only the bag had little effect on peroxide generation. In fat-free TPN solutions kept in covered bags, peroxide concentrations were 1.5 to 2 times higher when run through clear compared with colored tubings. When exposed to phototherapy or in the presence of lipids, peroxides were two to three times higher with the clear compared with the black tubing; meanwhile, orange and yellow tubings offered varying levels of protection related to their light-absorbing properties. Colored tubings offered a greater protection against the disappearance of thiol functions. Conclusions: Covering bags and using orange and yellow tubings may be a practical solution to reduce infused peroxide loads from about 400 to 100 µM. This is especially relevant in patients with an immature or a compromised antioxidant capacity or when phototherapy or preparations of TNA are used. (Journal of Parenteral and Enteral Nutrition 23:104-108, 1999)

Journal of Parenteral and Enteral Nutrition, Vol. 23, No. 2, 104-108 (1999)
DOI: 10.1177/0148607199023002104


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. Khashu, A. Harrison, V. Lalari, J.-C. Lavoie, and P. Chessex Impact of shielding parenteral nutrition from light on routine monitoring of blood glucose and triglyceride levels in preterm neonates Arch. Dis. Child. Fetal Neonatal Ed., March 1, 2009; 94(2): F111 - F115. [Abstract] [Full Text] [PDF]

				J. A. Rufian-Henares, E. Guerra-Hernandez, and B. Garcia-Villanova Evolution of Fatty Acid Profile and Lipid Oxidation During Enteral Formula Storage JPEN J Parenter Enteral Nutr, May 1, 2005; 29(3): 204 - 211. [Abstract] [Full Text] [PDF]

				J.-C. Lavoie, T. Rouleau, and P. Chessex Interaction between Ascorbate and Light-Exposed Riboflavin Induces Lung Remodeling J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 634 - 639. [Abstract] [Full Text] [PDF]