This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Iannoli, P. Articles by Sax, H. C. Search for Related Content PubMed PubMed Citation Articles by Iannoli, P. Articles by Sax, H. C. Social Bookmarking                         What's this?

Epidermal Growth Factor and Human Growth Hormone Induce Two Sodium-Dependent Arginine Transport Systems After Massive Enterectomy

Department of Surgery, University of Rochester Medical Center, Rochester, New York

Jen-nie H. Miller, MS

Department of Surgery, University of Rochester Medical Center, Rochester, New York

Harry C. Sax, MD

Department of Surgery, University of Rochester Medical Center, Rochester, New York

Background: A combination of epidermal growth factor (EGF) and human growth hormone (hGH) after massive enterectomy induces a 400% increase in arginine transport in the remnant distal small intestine. The kinetic mechanism (s) responsible for enhanced arginine transport under these conditions is unknown. Methods: New Zealand White rabbits underwent 70% midjejunoileal resection. After a 1-week recovery period, animals received hGH (0.2 mg/kg/d IM), EGF (1.5 µg/kg/h SC), hGH + EGF, or vehicle (equal volume) for 7 days. Transport of tritiated arginine into brush border membrane vesicles prepared from distal remnant small intestinal mucosa was quantified in the presence and absence of a sodium gradient over a range of arginine concentrations (25 to 5000 µmol/L). Results: Eadie-Hofstee transformation of the kinetic data demonstrates two sodium-dependent arginine transport systems, comprising a high-capacity, low-affinity system and a low-capacity, high-affinity system. A combination of EGF and hGH significantly upregulates both the high-capacity (685%) and low-capacity (350%) maximum transport velocity (V_max). Additionally, EGF alone significantly upregulates V_max by 200% in the low-capacity system. There were no significant changes in transport affinity (K_m) in either system. Conclusions: There are two quiescent sodium-dependent arginine transport systems in the distal small intestine. A combination of EGF and hGH after massive enterectomy increase arginine transport by V_max upregulation in both the high-capacity/low-affinity and lowcapacity/high-affinity systems. (Journal of Parenteral and Enteral Nutrition 22:326-330, 1998)

Journal of Parenteral and Enteral Nutrition, Vol. 22, No. 5, 326-330 (1998)
DOI: 10.1177/0148607198022005326


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. Grasemann, F. Ratjen, D. Schnabel, E. Reutershahn, U. Vester, and H. Grasemann Effect of growth hormone therapy on nitric oxide formation in cystic fibrosis patients Eur. Respir. J., April 1, 2008; 31(4): 815 - 821. [Abstract] [Full Text] [PDF]

				N. E. Avissar, T. R. Ziegler, L. Toia, L. Gu, E. C. Ray, J. Berlanga-Acosta, and H. C. Sax ATB0/ASCT2 Expression in Residual Rabbit Bowel Is Decreased after Massive Enterectomy and Is Restored by Growth Hormone Treatment J. Nutr., September 1, 2004; 134(9): 2173 - 2177. [Abstract] [Full Text] [PDF]

				J. S. Thompson Epidermal Growth Factor and the Short Bowel Syndrome JPEN J Parenter Enteral Nutr, September 1, 1999; 23(5_suppl): S113 - S116. [Abstract] [PDF]