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Effects of Nucleosides and a Nucleotide Mixture on Gut Mucosal Barrier Function on Parenteral Nutrition in Rats

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Toshimasa Tsujinaka, MD

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Masahiko Yano, MD

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Takashi Morimoto, MD

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Shohei Jima, MD

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Atsuhiro Ogawa, MD

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Hitoshi Shiozaki, MD

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Morito Monden, MD

Department of Surgery , Osaka University Medical School, Suita, Osaka, Japan

Background: We have previously reported that the addition of nucleosides and a nucleotide mixture (OG-VI) to total parenteral nutrition (TPN) prevents TPN-induced intestinal mucosal atrophy and results in increased intestinal cathepsin activities under TPN. The aims of the present study are to examine the effects of OG-VI on mucosal barrier functions and to clarify whether the intestinal cathepsins can be used as a new marker of mucosal barrier function. Method: Male Wistar rats were divided into three groups: FED (food ad libitum), TPN (a standard TPN solution), and OG (OG-VI in addition to the TPN solution). Mucosal barrier functions were analyzed by the lactulose/mannitol (L/M) test, mucosal ZO-1 messenger RNA level, and electron micrographs in the gut. Intestinal cathepsin B, H, and L activities were also measured. Results: The L/M ratio for TPN was much higher than that for FED. Only lactulose excretion was significantly reduced by OG-VI. The intercellular spaces of the mucosal cells for TPN were significantly wider than those for FED, but were reduced in size by the addition of OG-VI. All cathepsin activities of the ileum were higher for TPN than for FED, whereas OG showed no increase. Conclusion: OG-VI contributes to the improvement of gut mucosal barrier function. Intestinal cathepsin activities can be used as a new marker of mucosal barrier function. (Journal of Parenteral and Enteral Nutrition 21:104-111, 1997)

Journal of Parenteral and Enteral Nutrition, Vol. 21, No. 2, 104-111 (1997)
DOI: 10.1177/0148607197021002104


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