This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Boshi, Y. Articles by Okada, A. Search for Related Content PubMed PubMed Citation Articles by Boshi, Y. Articles by Okada, A. Social Bookmarking                         What's this?

Adhesive Mucous Gel Layer and Mucus Release as Intestinal Barrier in Rats

Department of Pediatric Surgery, Osaka University Medical School, Japan

Rchiro Nezu, MD

First Department of Surgery, Osaka University Medical School, Japan

Li Cui, MD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Kai Chen, MD, PHD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Jesmine Khan, MBBS

Department of Pediatric Surgery, Osaka University Medical School, Japan

Hiroshi Yoshida, MD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Kinya Sando, MD, PHD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Shinkichi Kamata, MD, PHD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Yoji Takagi, MD, PHD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Akira Okada, MD, PHD

Department of Pediatric Surgery, Osaka University Medical School, Japan

Background: Although it has been reported that total parenteral nutrition induces an increased intestinal permeability and a decreased mucous gel layer covering the intestinal epithelium, the role of mucous gel on intestinal permeability has not been well understood. We examined the in vivo effects of N-acetyl cysteine (NAC) as mucolytic agent and colchicine as suppressant of the mucus production on the intestinal transmission of fluorescein isothiocyanate dextran 70,000 (FITC-dextran). Methods: Rats were divided into four groups. In each group, FITC-dextran (750 mg/kg) with or without NAC (3000 mg/kg) was injected into the small intestinal lumen 3 hours after intraperitoneal injection of saline or colchicine (Col, 10 mg/kg). Thirty minutes after injection of FITC-dextran, blood samples were taken from portal vein to analyze plasma fluorescein concentration by fluorescence spectrometry. Samples of small intestine were sectioned in a cryostat for fluorescence microscopy, and the identical sections were stained by periodic acid-Schiff reaction. Results: Plasma FITC-dextran level in NAC group was higher than that in control group (p<.01), that in Col+NAC group was higher than that in Col group (p < .01) and that in Col + NAC group was higher than that in NAC group (p < .05). The spaces between villi were filled with mucous gel in the control and Col groups, whereas those were not entirely filled with mucous gel in NAC and Col + NAC groups. FITC-dextran and mucous gel showed complementary distribution in all rats. The villous interstitial edema was recognized in NAC group and the villi were disrupted in Col + NAC group. Conclusions: These results suggest that intestinal permeability is possibly affected not only by the mucous gel covering the intestinal epithelium but also by mucus release from goblet cells of the small intestine. (Journal of Parenteral and Enteral Nutrition 20:98-104, 1996)

Journal of Parenteral and Enteral Nutrition, Vol. 20, No. 2, 98-104 (1996)
DOI: 10.1177/014860719602000298


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				P. D. P. Lundin, M. Bojrup, H. Ljusberg-Wahren, B. R. Weström, and S. Lundin Enhancing Effects of Monohexanoin and Two Other Medium-Chain Glyceride Vehicles on Intestinal Absorption of Desmopressin (dDAVP) J. Pharmacol. Exp. Ther., August 1, 1997; 282(2): 585 - 590. [Abstract] [Full Text]

				Y. Boshi, R. Nezu, J. Khan, Kai Chen, Li Cui, H. Yoshida, M. Wasa, M. Fukuzawa, S. Kamata, Y. Takagi, et al. Developmental Changes in Distribution of the Mucous Gel Layer and Intestinal Permeability in Rat Small Intestine JPEN J Parenter Enteral Nutr, November 1, 1996; 20(6): 406 - 411. [Abstract] [PDF]