This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hinton, P. S. Articles by Ney, D. M. Search for Related Content PubMed PubMed Citation Articles by Hinton, P. S. Articles by Ney, D. M. Social Bookmarking                         What's this?

Insulin-Like Growth Factor-I Enhances Immune Response in Dexamethasone-Treated or Surgically Stressed Rats Maintained With Total Parenteral Nutrition

Department of Nutritional Sciences and School of Nursing, University of Wisconsin-Madison

Catherine A. Peterson, PHD

Department of Nutritional Sciences and School of Nursing, University of Wisconsin-Madison

Hui-Chen Lo, BS

Department of Nutritional Sciences and School of Nursing, University of Wisconsin-Madison

Huan Yang, MD, PHD

Department of Nutritional Sciences and School of Nursing, University of Wisconsin-Madison

Donna Mccarthy, PHD

Department of Nutritional Sciences and School of Nursing, University of Wisconsin-Madison

Denise M. Ney, PHD

Department of Nutritional Sciences and School of Nursing, University of Wisconsin-Madison

Background: New evidence suggests that insulin-like growth factor-I (IGF-I) is an important regulator of immune response. Our objective was to determine the effects of IGF-I on immune response during total parenteral nutrition (TPN) using two stress models. Methods: Male, Sprague-Dawley rats (230 to 250 g) were given TPN with or without coinfusion of recombinant human IGF-I (800 µg/d for 6 days) and subjected to either dexamethasone, a synthetic glucocorticoid, or surgical stress, in the form of a midline abdominal incision. In the dexamethasone model, immune response was assessed by total cellularity of the thymus and spleen, in vitro assays of lymphocyte proliferation, and interleukin 6 (IL-6) production, and concentrations of IL-6 and tumor necrosis factor (TNF-) in serum. In the surgical model, flow cytometry was used to identify and quantify splenic populations of T and B lymphocytes and macrophages. Results: In rats immunosuppressed by dexamethasone, IGF-I infusion increased mitogen-induced proliferation of thymocytes, but did not alter cellularity in the thymus; enhanced proliferation and IL-6 production in peripheral blood mononuclear cells following treatment with concanavalin A or lipopolysaccharide; and reduced the serum concentration of IL-6, but not TNF-. In surgically stressed rats, IGF-I infusion restored the splenic populations of immature and mature B lymphocytes, which were decreased by TPN. Conclusions: Our data demonstrate that IGF-I enhances immune response during TPN in rats. (Journal of Parenteral and Enteral Nutrition 19:444-452, 1995)

Journal of Parenteral and Enteral Nutrition, Vol. 19, No. 6, 444-452 (1995)
DOI: 10.1177/0148607195019006444


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K. R. Kritsch, S. Murali, M. L. Adamo, M. K. Clayton, and D. M. Ney Hypoenergetic High-Carbohydrate or High-Fat Parenteral Nutrition Induces a Similar Metabolic Response with Differential Effects on Hepatic IGF-I mRNA in Dexamethasone-Treated Rats J. Nutr., March 1, 2005; 135(3): 479 - 485. [Abstract] [Full Text] [PDF]

				P. S. Hinton, C. A. Peterson, E. M. Dahly, and D. M. Ney IGF-I alters lymphocyte survival and regeneration in thymus and spleen after dexamethasone treatment Am J Physiol Regulatory Integrative Comp Physiol, April 1, 1998; 274(4): R912 - R920. [Abstract] [Full Text] [PDF]