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Luminal Glutamine Perfusion Alters Endotoxin-Related Changes in Ileal Permeability of the Piglet

Department of Food Science and Nutrition, University of Alberta, Edmonton, Canada, Department of Animal Science. Texas University, College Station, TX 77843

Michael I. McBurney, PHD

Department of Food Science and Nutrition, University of Alberta, Edmonton, Canada

Background: The objective of this study was to investigate whether luminal perfusion with glutamine or with oxygenated glutamine solutions prevents endotoxin-induced changes in mucosal permeability. Methods: Three 15-cm segments of distal ileum were isolated in anesthetized 21-day-old piglets (n = 4) and perfused (50 mL/h) with Ringer's lactate solution, Ringer's lactate solution with 2% glutamine (wt/vol), glutamine, or glutamine purged with oxygen at 37°C for 280 minutes. Plasma-to-lumen clearances of ⁵¹Cr-EDTA and urea were measured to assess mucosal permeability. At time 0 minutes, loading and maintenance IV infusions of markers were begun. Baseline permeabilities were obtained from time 60 to 80 minutes, and IV endotoxin (50 µg/kg) was introduced from time 80 to 140 minutes. Results: Results are expressed as the ratio of the clearances of the two probes (C_EDTA/C_UREA). Permeability increased from baseline in loops perfused with Ringer's lactate solution vs loops perfused with glutamine purged with oxygen and with glutamine alone (p < .01). Saturation with oxygen was without effect inasmuch as glutamine alone negated permeability increases. Intestinal myeloperoxidase activity did not differ with perfusate (p > .05). Conclusions: These data suggest that endotoxin-induced permeability changes can be prevented or delayed by the supply of luminal glutamine at the time of insult. (Journal of Parenteral and Enteral Nutrition 19:83-87 1995)

Journal of Parenteral and Enteral Nutrition, Vol. 19, No. 1, 83-87 (1995)
DOI: 10.1177/014860719501900183


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