|
Sign In to gain access to subscriptions and/or personal tools.
|
Total Parenteral Nutrition Decreases Luminal Mucous Gel and Increases Permeability of Small Intestine
Yasuhiko Iiboshi, MD
Ruchiro Nezu, MD
Michelle Kennedy, MD
Makoto Fujh, MD
Masafush Wasa, MD
Masahiro Fukuzawa, MD, PHD
Shinhchi Kamata, MD, PHD
Yoji Takagi, MD, PHD
Akira Okada, MD, PHD
The distribution of fluorescein isothiocyanate dextran 70,000 (FITC-dextran) and mucous gel across the lumen of small intestine was observed as an investigation into the role of mucous gel on permeability in total parenteral nutrition (TPN). Thirty-two rats were randomly divided into two groups fed with either TPN or oral rat food. On day 4 or 7, FITC-dextran (750 mg/kg body weight) was given through the gastroduodenal tube. After 1 hour, blood samples were taken by aortic puncture to analyze plasma FITC-dextran by fluorescence spectrometry. Samples of small intestine with luminal contents were frozen and sectioned in a cryostat for fluorescence microscopy; the same sections were placed in a 0.2% celloidin solution for 3 minutes to preserve mucous gel and stained by periodic acid-Schiff reaction for light microscopy. The plasma level of FITC-dextran after 1 hour of this marker injection showed a significant increase (p < .01) in the TPN group compared with the rat food group on days 4 and 7. Morphologic findings on days 4 and 7 were similar in both the jejunum and ileum: The mucous gel filled the spaces between villi and FITC-dextran centered in the lumen in the rat food group, whereas the mucous gel decreased and FITC-dextran filled the spaces between villi in the TPN group. FITC-dextran and mucous gel showed complementary distributions in both groups. These data suggest that TPN decreases luminal mucous gel and increases permeability of small intestine in rats. (Journal of Parenteral and Enteral Nutrition 18:346–350, 1994)
Journal of Parenteral and Enteral Nutrition, Vol. 18, No. 4,
346-350 (1994)
DOI: 10.1177/014860719401800412
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
M. W. Schaart, A. C. J. M. de Bruijn, H. Schierbeek, D. Tibboel, I. B. Renes, and J. B. van Goudoever
Small intestinal MUC2 synthesis in human preterm infants
Am J Physiol Gastrointest Liver Physiol,
May 1, 2009;
296(5):
G1085 - G1090.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. van der Schoor, D. L Wattimena, J. Huijmans, A. Vermes, and J. B van Goudoever
The gut takes nearly all: threonine kinetics in infants
Am. J. Clinical Nutrition,
October 1, 2007;
86(4):
1132 - 1138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Faure, D. Moennoz, F. Montigon, C. Mettraux, D. Breuille, and O. Ballevre
Dietary Threonine Restriction Specifically Reduces Intestinal Mucin Synthesis in Rats
J. Nutr.,
March 1, 2005;
135(3):
486 - 491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Deplancke, O. Vidal, D. Ganessunker, S. M Donovan, R. I Mackie, and H R. Gaskins
Selective growth of mucolytic bacteria including Clostridium perfringens in a neonatal piglet model of total parenteral nutrition
Am. J. Clinical Nutrition,
November 1, 2002;
76(5):
1117 - 1125.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Deplancke and H R. Gaskins
Microbial modulation of innate defense: goblet cells and the intestinal mucus layer
Am. J. Clinical Nutrition,
June 1, 2001;
73
(6):
1131S - 1141S.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J M Mariadason, A Catto-Smith, and P R Gibson
Modulation of distal colonic epithelial barrier function by dietary fibre in normal rats
Gut,
March 1, 1999;
44(3):
394 - 399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ford and J. B. Pietsch
Home Enteral Tube Feeding in Children After Chemotherapy or Bone Marrow Transplantation
Nutr Clin Pract,
February 1, 1999;
14(1):
19 - 22.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Khan, Y. Iiboshi, L. Cui, M. Wasa, and A. Okada
Role of Intestinal Mucus on the Uptake of Latex Beads by Peyer's Patches and on Their Transport to Mesenteric Lymph Nodes in Rats
JPEN J Parenter Enteral Nutr,
January 1, 1999;
23(1):
19 - 23.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Kishibuchi, T. Tsujinaka, M. Yano, T. Morimoto, S. Jima, A. Ogawa, H. Shiozaki, and M. Monden
Effects of Nucleosides and a Nucleotide Mixture on Gut Mucosal Barrier Function on Parenteral Nutrition in Rats
JPEN J Parenter Enteral Nutr,
March 1, 1997;
21(2):
104 - 111.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Khan, Y. Boshi, R. Nezu, K. Chen, L. Cui, H. Yoshida, M. Wasa, M. Fukuzawa, S. Kamata, Y. Takagi, et al.
Total Parenteral Nutrition Increases Uptake of Latex Beads by Peyer's Patches
JPEN J Parenter Enteral Nutr,
January 1, 1997;
21(1):
31 - 35.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y. Boshi, R. Nezu, J. Khan, Kai Chen, Li Cui, H. Yoshida, M. Wasa, M. Fukuzawa, S. Kamata, Y. Takagi, et al.
Developmental Changes in Distribution of the Mucous Gel Layer and Intestinal Permeability in Rat Small Intestine
JPEN J Parenter Enteral Nutr,
November 1, 1996;
20(6):
406 - 411.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y. Boshi, R. Nezu, Li Cui, Kai Chen, J. Khan, H. Yoshida, K. Sando, S. Kamata, Y. Takagi, and A. Okada
Adhesive Mucous Gel Layer and Mucus Release as Intestinal Barrier in Rats
JPEN J Parenter Enteral Nutr,
March 1, 1996;
20(2):
98 - 104.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. L. Buchman, A. A. Moukarzel, S. Bhuta, M. Belle, M. E. Ament, C. D. Eckhert, D. Hollander, J. Gornbeln, J. D. Kopple, and S. R. Vijayaroghavan
Parenteral Nutrition Is Associated With Intestinal Morphologic and Functional Changes in Humans
JPEN J Parenter Enteral Nutr,
November 1, 1995;
19(6):
453 - 460.
[Abstract]
[PDF]
|
|
|
|
|
