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Direct Assay of Vitamins B1, B2, and B6 in Hospitalized Patients: Relationship to Level of Intake

Departments of Medicine, Clinical Nutrition-Research Unit, and Biomedical Engineering, Vanderbilt University School of Medicine, and the Department of Veterans Affairs Medical Center, Nashville, Tennessee

Julie Zimmer, BS

Departments of Medicine, Clinical Nutrition-Research Unit, and Biomedical Engineering, Vanderbilt University School of Medicine, and the Department of Veterans Affairs Medical Center, Nashville, Tennessee

Karen Meurer, MD

Departments of Medicine, Clinical Nutrition-Research Unit, and Biomedical Engineering, Vanderbilt University School of Medicine, and the Department of Veterans Affairs Medical Center, Nashville, Tennessee

Eric Manske, MD

Departments of Medicine, Clinical Nutrition-Research Unit, and Biomedical Engineering, Vanderbilt University School of Medicine, and the Department of Veterans Affairs Medical Center, Nashville, Tennessee

J.C. Collins, PHD

Departments of Medicine, Clinical Nutrition-Research Unit, and Biomedical Engineering, Vanderbilt University School of Medicine, and the Department of Veterans Affairs Medical Center, Nashville, Tennessee

Harry L. Greene, MD

Departments of Medicine, Clinical Nutrition-Research Unit, and Biomedical Engineering, Vanderbilt University School of Medicine, and the Department of Veterans Affairs Medical Center, Nashville, Tennessee

High-performance liquid chromatography assays for vitamins B₁ by erythrocyte thiamine pyrophosphate, B₂ by plasma and urinary riboflavin, and B₆ by plasma pyridoxal phosphate and urinary pyridoxic acid were used to evaluate the B vitamin status of hospitalized patients. Over an intake range of up to 3.4 mg of thiamine per day and up to 4.1 mg of riboflavin per day, erythrocyte thiamine pyrophosphate and urine and plasma riboflavin increased proportionately with intake. There was no relationship between B₆ intake and blood levels. Rather, a constant blood level was maintained with an intake range of 0.5 to 4 mg/d, and urinary pyridoxic acid showed a linear increase proportionate to intake. There were extremely variable blood and urine concentrations of B vitamins noted in our patient population. (Journal of Parenteral and Enteral Nutrition 17:315-316, 1993)

Journal of Parenteral and Enteral Nutrition, Vol. 17, No. 4, 315-316 (1993)
DOI: 10.1177/0148607193017004315


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