This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Jima, S. Articles by Mori, T. Search for Related Content PubMed PubMed Citation Articles by Jima, S. Articles by Mori, T. Social Bookmarking                         What's this?

Intravenous Administration of Nucleosides and a Nucleotide Mixture Diminishes Intestinal Mucosal Atrophy Induced by Total Parenteral Nutrition

Department of Surgery II, Osaka University Medical School, Japan

Toshimasa Tsujinaka, MD

Department of Surgery , Osaka University Medical School, Japan

Yoshihiro Kido, MD

Department of Surgery , Osaka University Medical School, Japan

Yoshihiko Hayashida, MD

Department of Surgery , Osaka University Medical School, Japan

Hideki Ishida, MD

Department of Surgery , Osaka University Medical School, Japan

Taro Homma, MD

Department of Surgery , Osaka University Medical School, Japan

Hiroomi Yokoyama, PHD

Nutrition Research, Otsuka Pharmaceutical Factory Inc, Tokushima, Japan

Takesada Mori, MD

Department of Surgery , Osaka University Medical School, Japan

Total parenteral nutrition (TPN) is associated with atrophic changes in the structure and function of the intestinal mucosa. Because rapidly renewing intestinal mucosal cells may require an external source of purines and pyrimidines for their optimal growth, it can be assumed that supplementation of nucleosides and a nucleotide mixture (OG-VI) during TPN may prevent the progression of mucosal atrophy by compensating for a relatively insufficient delivery from liver. To test that hypothesis, male Wistar rats receiving TPN for 7 days were divided into four groups according to different TPN solutions. Group C (n = 10) received a standard solution, group O (n = 10) received OG-VI in addition to the standard solution, and group G (n = 10) received a glutamine-rich TPN solution containing almost the same amount of calories and nitrogen as the standard solution. Group O+G (n = 10) received OG-VI in addition to the glutamine-rich solution. Various parameters were examined on the eighth day in the jejunal and ileal segments. The following significant changes in comparison with group C were observed: total wet weight of the jejunal segment in group O was significantly greater, as was mucosal wet weight of the jejunal and ileal segments in groups O and O+G; protein contents of the ileal segment in group O as well as the DNA content of the jejunal segment in group O increased significantly; and maltase activity of the jejunal segment in group O+G increased, as did the villus height of the jejunal segment in groups O and O+G and the villus height of the ileal segment in group G. Finally, proliferating cell nuclear antigen-positive cells in crypts of the jejunal segment in group O and group O+G increased significantly. The supplementation of OG-VI prevented the intestinal mucosal atrophic changes induced by TPN more effectively than did glutamine. The combined effect of OG-VI and glutamine was observed on the development of brush-border enzymes. Provision of an external source of purines and pyrimidines therefore has a supportive effect on intestinal mucosal growth during TPN. (Journal of Parenteral and Enteral Nutrition 17:265-270, 1993)

Journal of Parenteral and Enteral Nutrition, Vol. 17, No. 3, 265-270 (1993)
DOI: 10.1177/0148607193017003265


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. E. Evans, J. Tian, L. H. Gu, D. P. Jones, and T. R. Ziegler Dietary Supplementation With Orotate and Uracil Increases Adaptive Growth of Jejunal Mucosa After Massive Small Bowel Resection in Rats JPEN J Parenter Enteral Nutr, September 1, 2005; 29(5): 315 - 321. [Abstract] [Full Text] [PDF]

				T. Gross, R. Babst, A. Juretic, B. Herzog, P. Stehle, L. Filgueira, M. Oberholzer, F. Gudat, and M. Heberer Intralipid-Based Short-Term Total Parenteral Nutrition Does Not Impair Small Intestinal Mucosa-Related Cellular Immune Reactivity in the Healthy Rat JPEN J Parenter Enteral Nutr, November 1, 2000; 24(6): 337 - 344. [Abstract] [PDF]

				T. Tsujinaka, M. Kishibuchi, S. Iijima, M. Yano, and M. Monden Nucleotides and Intestine JPEN J Parenter Enteral Nutr, September 1, 1999; 23(5_suppl): S74 - S77. [Abstract] [PDF]

				R. McCauley, S.-E. Kong, and J. Hall Review: Glutamine and Nucleotide Metabolism Within Enterocytes JPEN J Parenter Enteral Nutr, March 1, 1998; 22(2): 105 - 111. [Abstract] [PDF]

				M. Kishibuchi, T. Tsujinaka, M. Yano, T. Morimoto, S. Jima, A. Ogawa, H. Shiozaki, and M. Monden Effects of Nucleosides and a Nucleotide Mixture on Gut Mucosal Barrier Function on Parenteral Nutrition in Rats JPEN J Parenter Enteral Nutr, March 1, 1997; 21(2): 104 - 111. [Abstract] [PDF]

				M. Kishibuchi, T. Tsujinaka, S. Jima, M. Yano, C. Ebisui, K. Kan, T. Morimoto, and T. Mori Interrelation of Intracellular Proteases With Total Parenteral Nutrition-Induced Gut Mucosal Atrophy and Increase of Mucosal Macromolecular Transmission in Rats JPEN J Parenter Enteral Nutr, May 1, 1995; 19(3): 187 - 192. [Abstract] [PDF]