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Comparative Effects of Thermal and Surgical Trauma on Rat Muscle Protein Metabolism

Department of Physiological Sciences, School of Veterinary Medicine

Bruce M. Wolfe, M.D.

Department of Surgery, School of Medicine

Robert J. Hansen, PH.D.

Department of Physiological Sciences, School of Veterinary Medicine

Andrew J. Clifford, PH.D.

Department of Nutrition, University of California, Dauis, California

A modified intraperitoneal pool flooding technique, employing L-³ H-tyrosine, was developed for measuring muscle protein synthetic rates following traumatic injury. Sufficient radiolabeled tyrosine was injected intraperitoneally to effect a six-fold increase in plasma tyrosine concentration (124-800 µM) resulting in constant, sustained specific radioactivities in plasma- and intracellular-free tyrosine pools. Localized vs systemic effects of thermal and surgical trauma on gastrocnemius muscle protein turnover were assessed 2 and 4 days postinjury. Thermal trauma increased total, myofibrillar, and sarcoplasmic muscle protein synthesis (44%) and protein degradation (300%). Conversely, surgical trauma decreased synthesis of total (24%), myofibrillar (14%), and sarcoplasmic (43%) muscle proteins without altering protein degradation. Short-term restriction of pair-fed controls did not affect either aspect of protein turnover. (Journal of Parenteral and Enteral Nutrition 15:128-136, 1991)

Journal of Parenteral and Enteral Nutrition, Vol. 15, No. 2, 128-136 (1991)
DOI: 10.1177/0148607191015002128


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