This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Just, B. Articles by Messing, B. Search for Related Content PubMed PubMed Citation Articles by Just, B. Articles by Messing, B. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CARBON DIOXIDE Social Bookmarking                         What's this?

Use of a Ventilated Canopy for Assessment of [13C]Leucine Oxidation in Patients Receiving Total Parenteral Nutrition

INSERM U.290, Paris

Dominique Darmaun, M.D.

INSERM U.290, Paris

Joseph Koziet, PH.D.

Pernod-Ricard Research Laboratory, Créteil, France

Monique Rongier, B.Sc.

INSERM U.290, Paris

Bernard Messing, M.D.

INSERM U.290, Paris

Rates of oxidation of infused ¹³C-labeled substrates are calculated from CO₂ production and ¹³C enrichment in breath CO ₂. Breath sampling through a mouthpiece is not appropriate in severely ill patients; the authors therefore validated the use of direct air sampling from the ventilated canopy of an indirect calorimeter for measuring the oxidation of ¹³C-labeled substrates. Infusions of H¹³CO₃ Na or L-[1-¹³C]leucine were performed in four healthy postabsorptive adults and six malnourished patients receiving total parenteral nutrition (TPN). At each sampling point, air was collected from the canopy to compare with breath air sampled through a mouthpiece and ¹³CO₂ enrichment determined by isotope ratio mass spectrometry. Despite five-fold dilution of expired air by room air within the canopy (a dilution required to maintain safe CO₂ levels in inspired air): (1) Breath ¹³CO₂ enrichment was accurately predicted using samples from the canopy, with a correction taking into account the measured CO₂ fractions in canopy and room air; (2) the precision in isotopic determination was similar with both methods (SD/mean of 12 determinations = 2.5 ± 1.0% us 3.0 ± 1.0%). These data demonstrate that the use of a ventilated canopy allows for combined assessment of energy expenditure and rates of oxidation of ¹³C-labeled substrates even in sick, debilitated patients receiving total parenteral nutrition. (Journal of Parenteral and Enteral Nutrition 15:65-70, 1991)

Journal of Parenteral and Enteral Nutrition, Vol. 15, No. 1, 65-70 (1991)
DOI: 10.1177/014860719101500165


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. A. Winter, S. J. O'Keefe, M. Callanan, and T. Marks The Effect of Severe Undernutrition and Subsequent Refeeding on Whole-Body Metabolism and Protein Synthesis in Human Subjects JPEN J Parenter Enteral Nutr, July 1, 2005; 29(4): 221 - 228. [Abstract] [Full Text] [PDF]

				P. Crenn, F. Thuillier, B. Rakatoambinina, M. Rongier, D. Darmaun, and B. Messing Duodenal vs. gastric administration of labeled leucine for the study of splanchnic metabolism in humans J Appl Physiol, August 1, 2000; 89(2): 573 - 580. [Abstract] [Full Text] [PDF]

				M. Dangin, J. C. Desport, P. Gachon, and B. Beaufrere Rapid and accurate 13CO2 isotopic measurement in whole blood: comparison with expired gas Am J Physiol Endocrinol Metab, January 1, 1999; 276(1): E212 - E216. [Abstract] [Full Text] [PDF]