This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Nunez, M. C. Articles by Gil, A. Search for Related Content PubMed PubMed Citation Articles by Nunez, M. C. Articles by Gil, A. Social Bookmarking                         What's this?

Effect of Dietary Nucleotides on Intestinal Repair in Rats with Experimental Chronic Diarrhea

Untasa Research Department, Granada

Maria V. Ayudarte, M.S.

Untasa Research Department, Granada

Daniel Morales, PH.D.

Untasa Research Department, Granada

Maria D. Suarez, PH.D.

Department of Biochemistry and Molecular Biology, University of Granada, Granada, Spain

Angel Gil, PH.D.

Untasa Research Department, Granada

Nucleic acid synthesis in tissues of rapid growth is preferentially done using dietary purines and pyrimidines via the salvage pathway. In the case of a low protein intake, dietary nucleotides may be semiessential for cell replication of gut, lymphocytes, and bone marrow, and especially in those intestinal diseases in which the mucosa is altered, dietary nucleotides may have a role in intestinal development. The effect of dietary nucleotides on intestinal weight and length, gut mucosal weight, intestinal protein and DNA contents, and lactase, maltase, and intestinal mucosal activities was assessed in a controlled way. Weanling (21-day-old) rats were separated into two groups of 36, each receiving blindly a basal diet containing glucose polymers (C) or a basal diet with lactose as the main carbohydrate (L) for 15 days. Those fed with L developed a syndrome of chronic diarrhea and malnutrition. Ten rats of each group were sacrificed at that time. The rest of the animals of each group were separated into two subgroups. The first was fed with the C diet and the second with the C diet supplemented with 50 mg/100 g of each of the following nucleotides: AMP, GMP, CMP, UMP, and IMP (CN). Thus the subgroups CC, CN, LC, and LN were formed. Rats were sacrificed after 4 weeks and gut separated into three segments corresponding to duodenum, jejunum, and ileum. Analysis of variance was used to compare the effect of diet or segments. DNA and lactase, maltase, and sucrase activities increased in the LN group with respect to LC especially in jejunum and ileum but there were not any differences between CC and CN. After malnutrition recovery, total proteins and DNA were similar in all groups. Dietary nucleotides appear to be modulators of intestinal development after chronic diarrhea and may have important implications in the growth of enterocyte. (Journal of Parenteral and Enteral Nutrition 14:598-604, 1990)

Journal of Parenteral and Enteral Nutrition, Vol. 14, No. 6, 598-604 (1990)
DOI: 10.1177/0148607190014006598


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Tsujinaka, M. Kishibuchi, S. Iijima, M. Yano, and M. Monden Nucleotides and Intestine JPEN J Parenter Enteral Nutr, September 1, 1999; 23(5_suppl): S74 - S77. [Abstract] [PDF]

				P. Sukumar, A. Loo, R. Adolphe, J. Nandi, A. Oler, and R. A. Levine Dietary Nucleotides Augment Dextran Sulfate Sodium-Induced Distal Colitis in Rats J. Nutr., July 1, 1999; 129(7): 1377 - 1381. [Abstract] [Full Text]

				J. Boza, O. Martinez, L. Baro, M. D. Suarez, and A. Gil Influence of Casein and Casein Hydrolysate Diets on Nutritional Recovery of Starved Rats JPEN J Parenter Enteral Nutr, May 1, 1995; 19(3): 216 - 221. [Abstract] [PDF]

				D. A. Novak, J. D. Carver, and L. A. Barness Dietary Nucleotides Affect Hepatic Growth and Composition in the Weanling Mouse JPEN J Parenter Enteral Nutr, January 1, 1994; 18(1): 62 - 66. [Abstract] [PDF]

				S. Jima, T. Tsujinaka, Y. Kido, Y. Hayashida, H. Ishida, T. Homma, H. Yokoyama, and T. Mori Intravenous Administration of Nucleosides and a Nucleotide Mixture Diminishes Intestinal Mucosal Atrophy Induced by Total Parenteral Nutrition JPEN J Parenter Enteral Nutr, May 1, 1993; 17(3): 265 - 270. [Abstract] [PDF]

				J. Boza, J. Jimenez, M. J. Faus, and A. Gil Influences of Postnatal Age and Dietary Nucleotides on Plasma Fatty Acids in the Weanling Rat JPEN J Parenter Enteral Nutr, July 1, 1992; 16(4): 322 - 326. [Abstract] [PDF]