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Effect of Emulsions of Medium and Long Chain Triglyceride on Human Adipose Tissue Prostaglandin Production in Vitro

Department of Pediatrics, Division of Nutrition and Metabolism, Department of Surgery, Mount Sinai School of Medicine, New York, New York

Jack Rudick

Department of Pediatrics, Division of Nutrition and Metabolism, Department of Surgery, Mount Sinai School of Medicine, New York, New York

Jerome L. Knittle

Department of Pediatrics, Division of Nutrition and Metabolism, Department of Surgery, Mount Sinai School of Medicine, New York, New York

Preliminary in vitro studies have been performed to assess the effect of experimental lipid emulsions of varying fatty acid composition on human adipose tissue metabolism. Subcutaneous human adipose tissue was obtained during elective surgery and placed in tissue culture. Physical mixtures of long chain triglyceride (LCT) and/or medium chain triglyceride (MCT) were added to the tissue culture medium so that the final concentration was 400 mg/dl. After a 3-day incubation period the tissue was harvested, placed in buffer and used to determine in vitro production of the prostaglandins prostacyclin I₂ (measured as its stable endproduct 6-keto PGF₁), thromboxane A₂ (measured as TXB₂), and prostaglandin E₂. Measurements of the fatty acid profile found in the neutral- and phospholipid fraction of the adipose tissue and fat cell size were also made. The results demonstrate that samples incubated in 100% MCT had the most significant increase in prostaglandin production whereas those incubated in 100% LCT had the most significant decrease in activity of the three prostaglandins assayed, when compared to controls. The addition of LCT to MCT caused a dose-related decrease in adipose tissue prostaglandin production. There were no significant changes in the profile of fatty acids found in the neutral- or phospholipid fraction of adipose tissue. The results indicate that the relative level of MCT/LCT incubated with human adipose tissue has a significant effect on prostaglandin production. (Journal of Parenteral and Enteral Nutrition 12:178-184, 1988)

Journal of Parenteral and Enteral Nutrition, Vol. 12, No. 2, 178-184 (1988)
DOI: 10.1177/0148607188012002178


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